BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b.

BD LSRFortessa™ X-20 Cell Analyzer Technical Specifications The BD LSRFortessa™ X-20 cell analyzer puts the power of the BD LSR platform into a compact footprint. It can easily fit on the benchtop for more cost-effective space utilization. The instrument delivers the optimal sensitivity and resolution required for multicolor applications.

The BD FACSDiva Software Reference Manual assumes that you have a working knowledge of basic Microsoft® Windows® operation. If you are not familiar About This Manual This manual describes how to use BD FACSDiva™ software. For information on how to operate and maintain your flow cytometer, refer to the instrument user’s guide. The BD FACSDiva Software Reference Manual assumes you have a working knowledge of basic Microsoft® Windows® operation.

Fortessa bd manual

2) Choose “New Experiment” . When acquiring the entire 96-well plate is better to have only have one plate per experiment. 3) Click new plate and choose the plate type. BD LSRFortessa X-20 Configuration Laser Detector Filter Mirror Dye 488nm (Blue) A 695/40 670LP PerCP, PerCP-Cy5.5 PerCP efluor 710 B 530/30 505LP FITC, GFP, CFSE, BB515 Alexa Fluor 488 C 488/10 - SSC 561nm (Yellow/Green) A 780/60 750LP PE-Cy7 B 670/30 635LP PE-Cy5, 7AAD, mPlum C 610/20 600LP PE-Texas Red, PE-CF594 BD LSRIIs and BD LSR-Fortessa have performance, capabilities, and flexibility never seen before in a benchtop analyzer.

Each software package has its own reference manual, provided on the documentation CD. BD Biosciences recommends that first-time users of this instrument take.

1 (Please refer to LSR Fortessa Standard Operation Protocol – Basic operation for Tube. Mode). Switching to The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers Could be equipped with BD High Throughput Sampler (HTS) option. BD LSR-Fortessa User's Guide · FACSDiva Software Reference Manu BD High Throughput Sampler User's Guide.

Nippon Becton Dickinson Company, Ltd. Tel 0120-8555-90 Asia Pacific Fax (65) 6-860-1590 Canada Tel (888) 259-0187 Fax (905) 542-9391 Tel (65) 6-861-0633 (905) 542-8028 canada@bd.com BD FACSDiva Software Reference Manual

If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then 1.844.8.bd.life (844.823.5433) Now with one customer support number, it's easier for you to review your order status, place a new order, get product information or learn about product availability. BD support quick links

Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential. BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) The facility at 2117A Everitt Lab (click link for campus map) is equipped with a BD LSR Fortessa analyser. It is very similar in operation to the LSR II cytometers (RAL and ERML Satelite Facilities) but with greater analysing sensitivity.
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Turn on the computer. Log in to Windows as the Operator. 2010-10-6 · BD FACSDiva Software Reference Manual. Simpler Gating Features † Proportional resizing of polygon and rectangular gates.
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25 Aug 2014 FORTESSA CONGIG 082514. Cytometer: Manual. Window Extension: 10.00. User: BDService. Institution: Software: BD FACSDiva 6.2. Date:.

A full tank provides about 3 hours’ run time. Refill as necessary from large plastic canister containing 1x sheath solution. If machine is on, depressurize 2015-4-9 · BD FACSDiva 6.1.2 – Tutorial USUHS Flow Cytometry Core Facility .

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The LSR Fortessa uses BD FacsFlow as the sheath fluid. 20 L boxes of which are available in lab 1.47a (CIMR) and on the bench behind the machine (e6) Please contact Flow Core Unit staff if none are present

11 Oct 2017 Web based software can aid in the panel design, such as BD Thus, mass cytometric data can be viewed and analyzed manually using standard as the LSR II® or LSR Fortessa® from Becton Dickinson (BD Biosciences, .